Nanographene oxide labeling with 188Re

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE

Nanographene oxide labeling with ¹⁸⁸Re

ZHANG Xiaoyong，

LI Jing，

ZHU Ying，

QI Yujin，

ZHU Zhiyong，

LI Wenxin，

HUANG Qing

Nuclear Science and Techniques

Vol.22, No.2

pp.99-104

Published in print 20 Apr 2011

DOI：10.13538/j.1001-8042/nst.22.99-104

36400

Nanographene oxide (NGO) is currently being explored for various biomedical applications. However, little information is known about its biological behaviors in vitro and in vivo. For further studying its pharmacokinetics and related biological behaviors in living systems, an effective and convenient tracing method is particularly demanded. In this work, NGO was labeled with radionuclide ¹⁸⁸Re (¹⁸⁸Re-NGO). To obtain high labeling yield and purity, a number of labeling conditions, including concentration of SnCl₂ and ascorbic acid, reaction time and temperature, and pH were optimized, and stability of the ¹⁸⁸Re-NGO in vitro and in vivo was evaluated. The results showed that NGO could be effectively labeled with high yield. The purified ¹⁸⁸Re-NGO showed high stability in vitro and in vivo. A pretest of NGO biodistribution with single photon emission computed tomography showed that the ¹⁸⁸Re-NGO was rapidly taken by organs such as lungs, liver, and spleen. The biodistribution of ¹⁸⁸Re-NGO differs significantly from the free radionuclide, indicating that the labeling procedure is highly suitable for investigating its biological behavior in living systems.

Graphene oxideRadio-labeling188ReSPECT imagesBiodistribution

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