Effect of N+ beam exposure on the activities of Mn-SOD and catalase in Deinococcus radiodurans

Effect of N⁺ beam exposure on the activities of Mn-SOD and catalase in Deinococcus radiodurans

SONG Dao-Jun ，

CHEN Ruo-Lei，

WU Li-Fang，

LI Hong，

YAO Jianming，

SHAO Chun-Lin，

WU Li-Jun，

YU Zeng-Liang

Nuclear Science and Techniques

Vol.11, No.4

pp.280-286

Published in print 01 Nov 2000

CLC：Q813.5, Q814, Q691.5

19900

Though the radiation-resistant bacteria Deinococcus radiodurans (D. radiodurans) have a high resistance to the lethal and mutagenic effects of many DNA-damaging agents, the mechanisms involved in the response of these bacteria to oxidative stress are poorly understood. In this report, the superoxide dismutase (SOD) and catalase (CAT) activities produced in bacteria (D.radiodurans AS1.633) and their change caused by 20keV N⁺ beam exposure were examined. Results showed that the activities of the enzymes were increased in the case of N⁺ beam exposure from 8 × 10¹⁴ ions/cm² to 6 × 10¹⁵ ions/cm². In addition, the treatment of H₂O₂ and [CHCl₃+CH₃CH₂OH] and the measurement of absorption spectrum showed that the increase of whole SOD activity resulted from inducible activities of Mn-SOD in (a sub-type) D.radiodurans AS1.633. These results suggested that these bacteria possess inducible defense mechanisms against the deleterious effects of oxidization.

N+ beam exposureDeinococcus radiodurans AS1.633Superoxide dismutaseCatalase

References

Brooks B W, Murray R G E. Int J Syst Bacteriol, 1981 31: 353~360

Masters C I, Minton K W. Plasmoid, 1992, 28: 258~261

Ghalib H A, Mackay M W et al. Gene, 1985, 33: 305~311

Huang Y, Anderson R. J Bacteriol, 1995, 177(9): 2564~2571

Tempest P R and Moseley B E B. Molec Gen Genet, 1980, 179: 191~199

Lennon E, Mintin K W. J Bacteriol. 1990, 172(6): 2955~2961

Daly M J, Ouyang L. J Bacteriol, 1994, 176(24): 7506~7515

Carroll J D, Daly M J, Minton K W. J Bacteriol, 1996, 178(1): 130~135

Daly M J, Ouyang L. J Bacteriol, 1994, 176(12): 35508~3517

Fridovich I. Science, 1978, 201: 875~880

Repine J E et al. Proc Natl Acad Sci U S A, 1981, 78: 1001~1003

Yu Z L et al. Nucl Instr Meth, 1991, B59/60: 705~708

Yu Z L et al. Nucl Instr Meth, 1993, B80/81: 1328~1331

Yu Z L. Radiat Phys Chem, 1994, 43(4): 349~351

Wang P. Can J Bacteriol, 1994, 41: 170~176

Chou F I, Tan S T. J Bacteriol, 1990, 172(4): 2029~2035

Yu Z L. Deng J G et al. Nucl Instr Meth, 1991, 59/60: 705~708

Yu Z L, Yang J B et al.

Studies of ion implantation effect on biology in China, China Nuclear Science and Technology Report (in Chinese), 1993 CNIC00746, ASIPP0036

Baidu Scholar

Google Scholar

Yu Z L, Yang J B et al. Nucl Instr Meth, 1993, B80/81: 1328~1331

Wang X Q, Han J W et al. Viva Origino, 1990, 26(2): 109~116

Beauchamp C, Fridiovich I, Anal Biochern, 1971, 44: 276~287

Beers R F, Sizer L W. J Biol Chem, 1952, 196: 133~140

Li P F, Fang Y Z. J Biochem Sinica, 1993, 417~423

Fang Y Z. Free radicals and enzyme and their application in biology and medicine. Beijing: Science and Technology Press, 1994, 71~109

Luo G H. Prog Biochem Biophys, 1996, 23(4): 356~359

Luo G H. Acta Phytophysiol Sinica, 1994, 10(2): 175~180