A solid phase radioimmunoassay for free triiodothyronine in serum:assay development and validation

LIU Fangyan，

ZHOU Meiying，

YIN Linxiang，

YANG Jianzhong，

HUA Chenglin

Nuclear Science and Techniques

Vol.10, No.1

pp.44-47

Published in print 01 Feb 1999

20000

A solid phase radioimmunoassay for free triiodothyronine in serum was developed based on double-antibody coated tubes. The method was turned out to be reliable with good reproducibility, higher sensitivity and easy performance. The measurable range of FT₃ in serum was 1.2 to 38 pmol/L. The mean coefficients of variation within and between assays were 1.79%~3.18% and 4.72%~9.31%, respectively. The FT₃ concentrations in euthyroid serum as determined by this method were 2.8 to 7.8 pmol/L. The FT₃ values determined by this new method correlated well with those measured by a commercial radioimmunoassay (r=0.853).

Free triiodothyronine (FT3)Solid-phaseRadioimmunoassayT3 analogDoubleantibody

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