Cellular and molecular studies of mutation induction by low energy heavy ions

Hei Tom K，

Zhu Li X，

Chen David J，

Waldren Charles A

Nuclear Science and Techniques

Vol.08, No.2

pp.107-113

Published in print 01 May 1997

19300

Mutation induction by low energy heavy ions was scored at the hypoxanthine guanine phosphoribosyl transferase (HGPRT) locus using both normal human fibroblasts and the human-hamster hybrid A_L cells. In addition, the mutation yield at a non-essential chromosome was also examined by using the S1 marker gene locating on human chromosome 11 in A_L cells. Mutagenicity induced by low energy heavy ions was dose and LET dependent. The induced mutant fractions at the S1 locus were consistently higher than those for HGPRT. Using a mutation system that can detect multilocus changes, it can be shown by either Southern blotting or multiplex PCR techniques that radiation can induce chromosomal deletions in the millions of basepairs.

MutationMolecular spectrumSouthern blotPCRMultilocus deletions

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