DETERMINATION OF TRACE ELEMENTS IN SUBCELLULAR FRACTION OF HUMAN LIVER BY INAA

Zheng Jian，

Zhuang Guisun，

Cheng Yuandi

Nuclear Science and Techniques

Vol.3, No.3

pp.216-221

Published in print 01 Aug 1992

40100

The concentrations of 18 elements in subcellular fractions of human liver were determined by combining differential centrifugation and INAA. Samples of human liver were homogenized in a buffer. The homogenate was separated into nuclei, mitochondrial, lysosomal, microsomal and cytosol fractions by successive differential centrifugation. Biological standard reference materials were used to evaluate the accuracy of the INAA method, and the results agree with the certified values. Element levels in subcellular fractions of human liver were discussed.

Trace elementsSubcellular fractionsINAA

REFERENCES

[1]

Ludany A. Kellermayer M. Jobst K. Acta Biochem Biophys Acad Sci Hung, 1980, 15: 229.

[2]

Sabbioni E. Gtrardi F. Sci Total Entiron, 1977, 7: 145.

[3]

Takeshi Saito. Toshihiro Itoh. Hiroshi Satoh et al. J Trace Elements in Experiment Medicine, 1988, 1: 33.

[4]

Rajan K S, Colbum R W. Davis J M. Life Science, 1976, 18: 423.

[5]

Jayawickreme CK. Chatt A. J Radioanal Nucl Chem, 1987, 110: 583.

[6]

Sabbioni E. Girardi F. Marafante E. Report EUR-5333. CEC. Joint Research Centre. Ispra Establishment. Italy, 1975.

[7]

Bradford M. Anal Biochem, 1976, 72: 248.

[8]

Cornelis R, Hoste J, Versieck J. Talanta, 1982, 29: 1029.

[9]

Currie L A. Anal Chem, 1968. 40: 586.

[10]

Novikoff A B. Holtzman E. Cells and organgelles. Philadelphia: Holt. Rinehart and Winston, 1976.

[11]

Iyengar G V. Kollmer W E. Bowen H J M. The elemental composition of human tissues and body fluids. New York: Verlag Chemie. Weinheim, 1978.

[12]

Montgomery R. Dryer R L. Conway T W et al. Biochemistry: a case-oriented approach. 4th ed. St Louis: The C V Mosby, 1983.

[13]

Anghileri L J. Int J Chn Pharmacol, 1976. 14: 101.

[14]

Dhar N K. Goel T C. Dube P C et al. Exp Mol Pathol, 1973, 19: 139.