LABELLING OF METALLOTHIONEIN WITH 99mTc

LABELLING OF METALLOTHIONEIN WITH ^99mTc

Li Bizhong，

Wu Yonghui，

Liu Yuanfang，

Li Jun，

Dong Yanbing，

Wang Chongqing，

Ru Binggen，

Han Bin，

Tian Jiahe

Nuclear Science and Techniques

Vol.3, No.4

pp.268-274

Published in print 01 Nov 1992

19500

^99mTc-labelled metallothionein (^99mTc-MT) was prepared through both direct and transcomplexation labelling approaches. Buffer systems and a variety of other parameters in the direct labelling procedure were studied in detail. High radiolabelling yields of 93-96% and the specific radioactivity of 3.7 MBg/µg were obtained under optimal conditions by the direct labelling method. The prepared ^99mTc-MT was stable in vitro. The chelate-exchange kinetics of MT with ^99mTc-glucohepatonate and ^99mTc-citrate were also studied. Biodistribution and imaging studies showed that ^99mTc-MT was accumulated in the animal kidneys at an exceptionally high level, indicating that ^99mTc-MT might be a potential renal imaging agent.

MetallothioneinTechnetium-99mRadiolabellingBiodistributionImaging

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