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RADIATION EFFECT ON BIOSYNTHESES OF ASCORBIC ACID AND RIBOFLAVIN IN GERMINATING CHICKPEA

RADIATION EFFECT ON BIOSYNTHESES OF ASCORBIC ACID AND RIBOFLAVIN IN GERMINATING CHICKPEA

Abdus Sattar
S. Atta
M. Wahid
B. Ahmad
M.A. Akhtar
Nuclear Science and TechniquesVol.1, No.4pp.222-228Published in print 01 Nov 1990
38700

The influence of irradiation on biosyntheses of ascorbic acid and riboflavin in germinating chickpea seeds at ambient (25— 35℃) conditions, was investigated. The rate of syntheses of these vitamins significantly increased with increasing germination time upto 120 h depending upon the treatment (P<0.05). Maximum amounts of ascorbic acid, 22.32 and 16.84 mg/100g, were found in the 0.10 kGy sample after 120 h of germination in tap and distilled water respectively. However, a radiation dose of 0.15 kGy resulted in the development of maximum values of riboflavin, 11.40 and 11.38μ g/g, on germination in tap and distilled water respectively. A significant linear relation(r=0.954 to 0.997) was observed between the biosyntheses of these vitamins and the germination time upto 120 h of irradiated and unirradiated chickpea seed (P<0.05).

IrradiationBiosynthesesAscorbic acidRiboflavin chickpea
REFERENCES
[1] R.J. Kutsky, Handbook of vitamins and hormones, Van Nostrand Reinhold Co., N.Y., 1973, p.51.
[2] K. Sax, Rad. Bot., 3 (1963), 179
[3] F.A. Loewus, Annal New York Acad. Sci. 92 (1961), 57.
[4] H. Mitsuda, Korean J. Food Sci. Tech., 3 (1971), 193.
[5] A. Sattar et al., Pak. J. Biochem, 22 (1989), in press.
[6] S. Bannerjee et al., Food Res., 19 (1954), 134.
[7] M.M. Gi- Lay and M.L. Fields, J. Food Sci., 46 (1981), 1069.
[8] R.D. King and P. Perwastien, J.Food Sci., 52 (1987), 106
[9] A.H.Y.A. Raman, Food Chem., 13 (1984), 17.
[10] A. Sattar et al., Food Chem., 17 (1985), 183.
[11] Anonymous , Fed. Register, 46 (1981), 59: 18992.
[12] A. Sattar et al., Food Chem., 34 (1989), 111.
[13] J. Augustin et al. eds. Methods of vitamin assay, Interscience Publishers, USA, 1985.
[14] J.M. Little and F.J. Hills, Agric. Extens. Univ. Calif. Davis., 1972.
[15] N.M. El- Shimi et al., Food Chem., 14 (1984), 11.
[16] A.M. Kylen and R.M. McCready, J.. Food Sci., 40 (1975), 1008.
[17] S.A. Warsi et al., Pak. J. Biochem, 10 (1977), 55.
[18] K. Uchida and Y. Suzuki, Agric. Biol. Chem., 38 (1974), 195.
[19] Neelofar Ph. D. Thesis, Univ. Peshawar, Pakistan, 1987.