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Nano-manipulation of single DNA molecules

NUCLEAR, HEAVY ION AND ATOMIC PHYSICS

Nano-manipulation of single DNA molecules

HU Jun
Jun-hong
LI Hai-kuo
AN Hong-jie
WANG Guo-hua
WANG Ying
LI Min-qian
ZHANG Yi
LI Bin
Nuclear Science and TechniquesVol.15, No.3pp.140-143Published in print 01 Jun 2004
22400

Nano-manipulation of single atoms and molecules is a critical technique in nanoscience and nanotechnology. This review paper will focus on the recent development of the manipulation of single DNA molecules based on atomic force microscopy (AFM). Precise manipulation has been realized including varied manipulating modes such as "cutting" , "pushing" , "folding" , "kneading" , "picking up" , "dipping" , etc. The cutting accuracy is dominated by the size of the AFM tip, which is usually 10nm or less. Single DNA fragments can be cut and picked up and then amplified by single molecule PCR. Thus positioning isolation and sequencing can be performed.

Nano-manipulationSingle DNA moleculesAtomic force microsco pyMolecular combingMolecular cutting
References
[1] Namasivayam V, Larson R G, Burke D T et al. Anal Chem, 2003, 75: 4188
[2] J H, Wu S Y, Wang G H et al. J Chin Electro Microsc Soc, 2003, 22: 185
[3] Mizuno A, Katsura S. J Bio Phys, 2002, 28: 587
[4] Ruano G, Kidd K K, Stephens J C. Proc Natl Acad Sci USA, 1990, 87: 6296
[5] Ding C, Cantor C R. Proc Natl Acad Sci USA, 2003, 100: 7449
[6] Eigen M, Rigler R. Proc Natl Acad Sci USA, 1994, 91: 5740
[7] Eigler D M, Schweizer E K. Nature, 1990, 344: 524
[8] Eigler D, Lutz M C P, Rudge W E. Nature, 1991, 352: 600
[9] Bartels L, Meyer G, Rieder K H. Phys Rev Lett, 1997, 79: 697
[10] Meyer G, Neu B, Rieder K H. Appl Phys A, 1995, 60: 343
[11] Jung T A, Schlittler R R, Gimzewski J K et al. Science, 1996, 271: 181
[12] Stipe B C, Rezaei M A, Ho W. Science, 1998, 279: 1907
[13] Meyer G, Rieder K H. Surf Sci, 1997, 377-379: 1087
[14] Decossas S, Patrone L, Bonnot A M et al. Surf Sci, 2003, 543: 57, and references therein
[15] Thalhammer S, Geigl J, Zink A et al. Acta Microscopica (Suppl), 2003, 12: 1, and references therein
[16] Piner D, Zhu J, Xu F et al. Science, 1999, 283: 661
[17] Fotiadis D, Scheuring S, Müller S A et al. Micron, 2002, 33: 385
[18] Thalhammer S, Stark R W, Müller S et al. J Struct Biol, 1997, 119: 232
[19] Stark R W, Thalhammer S, Wienberg J et al. Appl Phys A, 1998, 66: S579
[20] Xu X M, Ikai A. Anal Chim Acta, 1998, 361: 1
[21] Guthold M, Cubicciotti R, Superfine R et al. Biophys J (Annual Meeting Abstracts), 2002, 82: 163
[22] Osada T, Uehara H, Kim H et al. J Nanobiotech, 2003, 1: 2
[23] Bensimon A, Simon A, Chiffaudel A et al. Science, 1994, 265: 2096
[24] Ouyang Z, Hu J, Chen S F et al. J Vac Sci Technol B, 1997, 15: 1385
[25] Hu J, Wang M H, Weier U G et al. Langmuir, 1996, 12: 1697
[26] Hu J, Zhang Y, Gao H B et al. Nano Lett, 2002, 2: 55
[27] Lagally E T, Medintz I, Mathies R A. Anal. Chem, 2001, 73: 565
[28] Li H, Xue G, Yeung E S. Anal Chem, 2001, 73: 1537
[29] Nakano M, Komatsu J, Matsuura S et al. J Biotechnol, 2003, 102: 117
[30] Ohuchi S, Nakano H, Yamane T. Nucleic Acids Res, 1998, 26: 4339
[31] Mitra R D, Church G M. Nucleic Acids Res, 1999, 27: e34
[32] Wabuyele M B, Soper S A. Single Mol, 2001, 1: 13
[33] Nakano H, Kobayashi K, Ohuchi S et al. J Biosci Bioeng, 2000, 4: 456
[34] Rungpragayphan S, Kawarasaki Y, Imaeda T et al. J Mol Biol, 2002, 318: 395
[35] Grace M, Buzard G S, Hughes M R et al. Anal Biochem, 1998, 263: 85
[36] Li B, Wang Y, Wu H P et al. Chin Sci Bull, 2004, 49(7): 665-667