Preparation of silica-coated CdSe QDs for cell imaging applications

LOW ENERGY ACCELERATORS AND RADIATION APPLICATIONS

Preparation of silica-coated CdSe QDs for cell imaging applications

XIE Chunjuan，

LI Jian，

YIN Dongguang ，

ZHANG Li，

LIU Binhu，

WU Minghong

Nuclear Science and Techniques

Vol.21, No.2

pp.80-83

Published in print 20 Apr 2010

DOI：10.13538/j.1001-8042/nst.21.80-83

40100

CdSe quantum dots (QDs) were prepared at room temperature by E-beam irradiation of the solution, using ethylene diamine tetraacetic acid as stabilizer. Silica-coated CdSe QDs were made in water-in-oil microemulsion, while amino groups were introduced synchronously onto the nanoparticles by copolymerization of 3-aminopropyl- trimethoxysilane and tetraethyl orthosilicate. The results of TEM imaging, fluorescent spectroscopy and photostability tests show that the QDs are of uniform spherical size (200±8 nm), monodispersity, and high fluorescence and photostability.

E-beamsFluorescent quantum dotsMicro-spheresReverse microemulsionCell labels

References

[1]

Taton T A, Mirkin C A, Letsinger R L, et al. Science, 2000, 289: 1757-1760.

[2]

Nam J M, Thaxton C S, Mirkin C A, et al. Science, 2003, 301: 1884-1886.

[3]

Zhang S S, Zhong H, Ding C F. Anal Chem, 2008, 80: 7206-7212.

[4]

Bruche M, Moronne J M, Gin P, et al. Science, 1998, 281: 2013-2016.

[5]

Choi J H, Chen K H, Strano M. J Am Chem Soc, 2006, 128: 15584-15585.

[6]

Peng H, Zhang L J, Soeller C. J Am Chem Soc, 2007, 129: 3048-3049.

[7]

Cui D, Pan B F, Zhang H. Anal Chem, 2008, 80: 7996-8001.

[8]

Gao H, Zhao Y, Fu S, et al. Colloid Polym Sci, 2002, 280: 653-660.

[9]

Santra S, Zhang P, Wang K, et al. Anal Chem, 2001, 73: 4988-4993.

[10]

Zhao X, Hilliard L R, Mechery S J, et al. J Am Chem Soc, 2003, 125: 11474-11475.

[11]

Ferrari M. Nat Rev Cancer, 2005, 5: 161-171.

[12]

Lyons, S K. J Pathol, 2005, 205: 194-205.

[13]

Belloni J, Delcourt M O, Leclere C. Nouv J Chim,1982, 6: 507-509.

[14]

Marignier J L, Belloni J, Delcourt M O, et al. Nature, 1985, 317: 344-346.

[15]

Henglein A, Mulvaney P, Linnert T. Faraday Discuss, 1991, 92: 31-44.

[16]

Khatouri J, Mostafavi M, Amblard J, et al. Chem Phys Lett, 1992, 191: 351-356.

[17]

Mostafavi M, Keghouche N, Delcourt M O. Chem Phys Lett, 1990, 169: 81-84.

[18]

Zhu Y J, Qian Y T, Li X J, et al. Chem Commun, 1987, 12: 1081-1082.

[19]

Ye Z Q, Tan M Q, Wang G L. Anal Chem, 2004, 76: 513-518.

[20]

Buxton G V, Farhataziz I, Rodgers M A J. Editors, Radiation Chemistry: Principles and Applications, VCH, New York 1987.

[21]

Yang Q, Tang K B, Wang F, et al. Mater Lett, 2003, 57: 3508-3512.

[22]

Yin Y D, Xu X L, Ge X W, et al. Radiat Phys Chem, 1998, 53: 567-570.

[23]

Henry A C, Tutt T J, Galloway M, et al. Anal Chem, 2000, 72: 5331-5337.