Microarray screening of differentially expressed genes in DCX transfected U87 cells before and after irradiation

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE

Microarray screening of differentially expressed genes in DCX transfected U87 cells before and after irradiation

FENG Shuang，

WANG Jie，

JIANG Xin，

XU Yuanyuan，

ZHANG Haowen，

Cao Jianping，

YU Jiahua，

LIU Fenju

Nuclear Science and Techniques

Vol.23, No.2

pp.97-102

Published in print 20 Apr 2012

DOI：10.13538/j.1001-8042/nst.23.97-102

32500

Radiation therapy plays a critical role in the treatment of neurogliocytoma and it is known that doublecortin (DCX)-transfected U87 cells can inhibit tumor cell growth. Microarray analysis to screen for differentially expressed genes in DCX-transfected U87 cells before and after radiation uncovered DCX-related genes, the functions of DCX, and downstream genes in radiation therapy of neurogliocytoma. Stably transfected U87 cells were constructed (DCX-U87) and the differentially expressed genes were screened by microarray analysis to compare U87 cells with DCX-U87 cells in both non-irradiated and irradiated conditions. Cells were irradiated using ⁶⁰Co γ-ray at a dose rate of 1.0 Gy/min. Mean values were subject to paired comparison analysis and genes with a p-value of less than 0.05 were analyzed. Differentially expressed genes can correlate with radiation sensitivity and DCX transfection. DCX and SPN proteins in DCX-U87 cells were detected by two groups of 0 and 10 Gy, but not the U87 cells, and their expression levels were higher in the 10 Gy group than in the 0 Gy group. The differential gene expression in DCX-U87 cells before and after radiation is helpful for future investigations into the mechanisms of radiation therapy in neurogliocytoma cells.

MicroarrayDCXU87Ionizing radiation

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