Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I

INTERDISCIPLINARY STUDIES

Effects of nanoparticles on fluorescence enhancement of the complexes of dsDNA and SYBR Green I

CHEN Jiajia，

ZHANG Chen，

ZHAO Binyu，

MI Lijuan，

ZHANG Yi，

HU Jun

Nuclear Science and Techniques

Vol.23, No.4

pp.247-251

Published in print 20 Aug 2012

DOI：10.13538/j.1001-8042/nst.23.247-251

31100

Fluorescence enhancement in a DNA-dye system is favorable for sensitive and accurate DNA detection/ sensing technologies. In this paper, we report that the fluorescence of the double-stranded DNA (dsDNA) and SYBR Green I (SG) system (dsDNA-SG) can be effectively enhanced by negatively charged magnetic iron oxide (Fe₂O₃@DMSA) and gold nanoparticles in suitable concentrations, but positively charged nanoparticles quench the fluorescence. Effects of the Fe₂O₃@DMSA on the fluorescence intensities are investigated with dsDNA-SG of different lengths or complexities. The results show that nanoparticles perform similarly in enhancing fluorescence intensity for several kinds of dsDNA. However, the dsDNA concentration determines the fluorescence amplitude. It shows that fluorescence intensity of lower concentration dsDNA is enhanced remarkably in DNA-SG. The finding may be useful in sensitive biomolecular detection.

Surface chargesFluorescence enhancementNanoparticlesDouble-stranded DNASYBR Green I

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