Atomic force microscopy study on eucaryotic ribosomes

Xu Lei，

Wu Xiao-Hua，

Liu Wang-Yi，

Li Min-Qian

Nuclear Science and Techniques

Vol.07, No.01

pp.10-15

Published in print 01 Feb 1996

54100

It is tried to compare the effects of sample preparation on shapes and sizes of ribosomes deposited on mica. The apparent sizes of ribosomes in the atomic force microscopy (AFM) images are about 60 nm in most cases. The height appears to be indifferent to the sample preparation method and image conditions. It is found that the dehydration may greatly affect the topography of ribosomes. The volumes of ribosomes are estimated after considering the convolution effects of AFM tip on the appearance of ribosomes.

Atomic froce microscopoyEucaryotic ribosomeTopographySizeVolumeRat liverAmbient condition

References

Unwin P N T. Nature, 1977; 269: 118

Yonath A, Wittmann H G. Biophys Chem, 1988; 29: 17

Yonath A, Leonard K R, Wittmann H G. Science, 1987; 236: 813

Redermacher M, Wagenknecht T, Versschoor A et al. J Microsc, 1987; 146: 113

Frank J, Radermacher M, Wachenknecht T et al. Methods Enzymol, 1988; 164: 3

Frank J, Vershoor A, Radermacher M et al. Trends Biochem Sci, 1988; 13: 123

Lake J A. Ann Rev Biochem, 1958; 54: 507

Yonath A, Bennett W, Weinstein W et al. In: Hill EW, Dahlberg A, Garret RA et al, eds. The ribosomes, structure, function and evolution. Washington D C: Am Soe Microbiol. 1990; 234

Binnig G, Quate C F, Gerber G. Phys Rev Lett, 1986; 56: 930

Bustamante C, Keller D, Yang G, Current Opinion in Structural Biology, 1993; 3: 353

Li M Q, Hansma H G, Vesenka J et al. J Biomol Struct and Dynam, 1992; 10: 607

Li M Q, Hansma H G, Hong G F et al. J Vac Sci Technol, 1994; 12: 1465

Hansma H G, Sinsheimer R S, Groppe J et al. Scanning, 1993; 15: 286

Hansma H G. Annu Rev Biophys Struct, 1994; 23: 115

Hansman H G, Laney D L, Besanilla M et al. Application for atomic force microscopy of DNA (1994), preprint

Spedding G.

Isolation and analysis of ribosomes from prokaryotes, enkaryotes and organelles

. In: Spedding G, ed. Ribosomes and protein synthesis, a practical approach. London: IRL Press, 1990: 1

Baidu Scholar

Google Scholar

Lyubchenko Y L, Shlyakhtenko L S, Harrington R E et al. PRoc Natl Acad Sci, 1993; 90: 2137

Besanilla M, Bustamante C, Hansma H G. Scanning Microscopy, 1993; 7: 1145

Lake J A. Sci Am, 1981; 145: 84

Boublik M, Oostergetel G T, Wall T S et al.

Structure of ribosomes and their components by advanced techniques of electron microscopy and computer image analysis

. In: Hardesty B, Hramer G, eds. Structure, function and genetics of ribosome. Springer-Verlag New York Inc, 1986: 68

Baidu Scholar

Google Scholar

Engel A, Meyer J. J Ultrastract Res, 1980; 72: 212

Frank J, Penczek P, Grassucci R et al. J Cell Biol, 1991; 115: 597

Wagenhnecht T, Carazo J M, Radermacher M et al. Biophys J, 1989; 55: 455