AFM studies of nonspecific binding of enzyme on DNA

Zhang Yi，

Xu Lei，

Ouyang Zhen-Qian，

Chen Sheng-Fu，

Sun Jie-Lin，

Hu Jun，

Li Min-Qian，

Xie Heng-Yue，

Liu Zheng-Xia

Nuclear Science and Techniques

Vol.7, No.3

pp.151-153

Published in print 01 Aug 1996

37800

Atomic force microscope (AFM) is used to study restriction endonuclease digestion of plasmid DNA. pWRr plasmid DNA is digested by Hind III, and the specific and the nonspecific binding of the restriction endonuclease are imaged, and the biological function of the enzyme binding to nonspecific sites is discussed. In addition, it is found that nonspecific binding of Hind III could not induce the DNA characteristic bending angle.

Atomic force microscopePlasmid DNARestriction endonucleaseNonspecific binding

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