STIMULATING EFFECT OF LOW DOSE 147Pm ON DNA REPAIR IN SPERMIOGENIC STAGES

STIMULATING EFFECT OF LOW DOSE ¹⁴⁷Pm ON DNA REPAIR IN SPERMIOGENIC STAGES

Zhu Shoupeng，

Wang Liuyi

Nuclear Science and Techniques

pp.18-21

Published in print 01 Feb 1995

51300

For labelling spermatid DNA, male BALB/c mice are injected intratesticularly with ³H-TdR in advance. After 36 d of labelling, spermatozoa are sampled which is just the time required for the germ cells to mature. The period from intraperitoneal injection of ¹⁴⁷Pm to sampling spermatozoa is 12d. After sperm cells have been lysed, 30 ml eluent is added by wriggle pump. Finally, both the DNA remained on the filters and filtrated bottles are determined with a Beckman liquid scintillation device. Results show that after small dose internal irradiation with 185 Bq/g of ¹⁴⁷Pm, an increase in amount of sperm DNA on the filter observed is considerably higher than the control group. This indicates that low level internal radiation of ¹⁴⁷Pm has tendency to stimulate the DNA repair in spermiogenic stages.

Stimulating effect147PmLow dose irradiationDNA repairSpermiogenic stagesInternal irradiationMice

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