CONFORMATION STUDY OF THE LARGE FRAGMENT OF E. COLI DNA POLYMERASE I BY STM

Yao Xiaowei，

Li Minqian，

Xu Yaoliang，

Gu Minming，

Hu Jun，

Zhang Lanping，

Zhang Jiahua，

Lu Changde，

Qiu Minyan

Nuclear Science and Techniques

Vol.3, No.4

pp.260-262

Published in print 01 Nov 1992

21400

The large fragment of E.coli DNA polymerase I is imaged by scanning tunneling microscope. The specimen is deposited on highly oriented pyrolytic graphite surface, and then covered with pure paraffin oil in order to maintain hydration of the molecules. Images of the enzyme reveal an ellipsoid shape of 5.5-6.0 nm wide and 7.0-7.5 nm long. The conformation of the enzyme is in agreement with the model derived from X-ray crystallography studies.

STME. coli DNA polymerase I

REFERENCES

[1]

Beese L S, Seitz T A. In: Eckstein F, Lilley D M J eds. Nucleic acids and molecular biology. Heildeberg: Spring-Verlag Berling, 1989. 3: 28.

[2]

Li Minqian, Zhu Jinde, Zhu Jieqing et al. In: Zbarsky I B, Harris J R eds. Nuclear structure and function. London: Plenum Pub Co, 1990. 285.

[3]

Brick P, Ollis D, Steitz T A. J Mol Biol, 1985. 166: 453.

[4]

Steitz T A, Weber I T, Ollis D et al. J Biomolec Struct Dyn, 1983, 1: 1023.

[5]

Warwicker J, Ollis D, Richards F M et al. J Mol Biol, 1985, 186: 645.

[6]

Joyce C M, Steitz T A. Trends in Biochemical Science, 1987, 12: 288.

[7]

Clemmer C R, Beebe T P. Science, 1990, 251: 640.