Nuclear Science and Techniques

A PIXE METHOD TO STUDY BIOLOGY AT THE DNA LEVEL

Sun Yongnian，

Zeng Xianzhou，

Wang Xunming，

Huang Zuen，

Wu Xiankang，

Yang Fujia，

C.C. Tan

Nuclear Science and Techniques

Vol.1, No.4

pp.198-202

Published in print 01 Nov 1990

3600

An external proton beam system with a detection sensitivity of 10^-11g for ruthenium was used to study DNA. A metal- organic compound tris (4,7- diphenylphenanthroline) ruthenium (Ⅱ) [ $R u {(D i P)}_{3}^{2 +}$ ] was chosen as a metal marker. The target DNA labeled with $R u {(D i P)}_{3}^{2 +}$ was electrophoresed in an agarose gel and then was analysed by PIXE. The DNA sample with the metal marker showed clearly Ru peaks in the PIXE spectra, while the control showed no Ru peak at all. This method can be used to study biology at the DNA level.

Deoxyribonucleic acid (DNA)Tris (4,7- diphenylphenanthroline) ruthenium (Ⅱ)ElectrophoresePIXE

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