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The human granulocytes were isolated. Using hybridoma techniques, a hybridoma cell line (HSN) producing monoclonal antibody (McAb) against human granulocyte was obtained. The antibody belonged to IgG1 subclass. It was confirmed by immunohistochemical tests that HSN reacted selectively not only with human granulocytes, but also with their bone marrow precursors. Whereas human lymphocytes and red blood cells retained negative in the tests. No cross-reaction was observed with the peripheral blood cells in other animals. Its affinity constant was 5.7×10⁸L/mol, and the number of epitopes per granulocyte was 4.7×10⁵. Monoclonal antibody displayed no loss of immunoreactivity after labelled with ^99mTc.

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Two kinds of rhenium labeled radiopharmaceuticals for therapy of the human brain glioma were prepared. Anti-human brain glioma monoclonal antibody (McAb-SZ39) was directly labeled with186ReO4−. When ascorbic acid was used as reduction agent of antibody and the reduction of186ReO4− was finished in sodium gluconate solution (pH=2.54), the labeling efficiency was 96.2%. After purification, the labeled products were tested in respect of immunoreactivity, in vitro stability as well as inhibitory efficiency against human glioma transplanted in nude mice.188ReO4− labeled colloid was synthesized by using a two-step method. The size of radiocolloid particles was around 2~5μm, and the radiochemical yield was over 95%. Nude mice Experiments demonstrated that the inhibitory efficiency against human brain glioma of ¹⁸⁸Re-colloid and ¹⁸⁸Re-McAb-SZ39 was 90% and 65%, respectively.

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In advancing gastric cancer, especially when the serous is invaded, the plantation of cancer cells in peritoneal is common and it affects patients' survival time severely. Based on successfully labeled McAb (monoclonal antibody) 3H11 with ¹⁸⁸Re, we investigated the effect of RIT (Radioimmuno-Therapy) with ¹⁸⁸Re-3Hll on preventing the peritoneal micrometastasis of gastric cancer cells in nude mice to increase the survival time. After 1×10⁶ BGC-823 gastric cancer cells were injected into the peritoneal cavity of each mouse, 45BABL/C nude mice were divided into 9 groups. Each group received different doses of ¹⁸⁸Re-3Hll or ¹⁸⁸Re-IgG, or saline I.P.16 hours postoperation. The injected volume of each mouse was 1.0mL. The results showed that the survival time depended on the injected doses during 0 to 37 MBq. The survival time was 170±25.3d after 37 MBq ¹⁸⁸Re-3H11 were treated. It was over 5 times more than that for the saline group and about 3 times more than that for 37 MBq ¹⁸⁸-Re IgG group (p<0.05). The mice hemogram were reduced to lowest 14 days after injection, but they recovered after 28 d. Conclusion: with proper injection doses,early postoperative ¹⁸⁸Re-3H11 I.P. is effective and safe for the prevention of intra-peritoneally injected gastric cancer cells from surviving,growing and disseminating in nude mice.

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The syntheses and HPLC analysis of N-desmethyltamoxifen and carbon-11 labelled tamoxifen are described. In order to obtain the N-desmethyltamoxifen, tamoxifen citrate was first converted to tamoxifen free base. N-desmethyltamoxifen was prepared by reacting tamoxifen free base with 1-chloroethyl-chloroformate(ACE.Cl). For ¹¹C labeling, N-desmethyltamoxifen was heated with ¹¹C methyl iodide for 10min at 130°C, and the ¹¹C labelled compound was purified by HPLC on a μBonapak TM C₁₈ column. Injectable ¹¹C-tamoxifen was obtained within 50~60 min from EOB (end-of-bombardment) with a labeling yield of 60%~70%.

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A comparison of ^99mTc- CQDO and ^99mTc-CQDO-MeB has been made for biodistribution and pharmacokinetics. ^99mTc-CQDO and its adducts of methaneboronic acid ^99mTc-CQDO-MeB were prepared by the reduction of Na^99mTcO₄ with SnCl₂·2H₂O in aqueous solution. Radiochemical purity of ^99mTc-CQDO and ^99mTc-CQDO-MeB determined by TLC were over 95% after extraction. Biodistri-butions of ^99mTc-CQDO and ^99mTc-CQDO-MeB in mice demonstrated that both of them could be easily absorbed by myocardium, and the peak uptake of each were 10.83±2.2% ID/g and 11.84±1.69%ID/g, respectively. ^99mTc-CQDO showed rapid clearance from myocardial tissue while ^99mTc-CQDO-MeB had long retention in heart muscle. The myocardial uptake of ^99mTc-CQDO was only 5.88±1.66%ID/g at 10min. and the uptake of ^99mTc-CQDO-MeB was 7.42±0.17%ID/g at 60 min. The elimination of each from blood has a biexponential pattern, the first T_1/2 is 1.38 and 1.5 min, respectively. The partition coefficient of ^99mTc-CQDO and ^99mTc-CQDO-MeB were 20 and 25 at pH 7.40, respectively.

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In order to understand the molecules mechanism of ion irradiation, which has been widely used in many fields such as cancer therapy, uracil, one of the bases of nucleic acid, was chosen in the low energy ion radiolysis research. The solid uracil films with mass thickness of 0.314 mg/cm² were irradiated by 200 keV H⁺ ions. The experimental results show that 200 keV H⁺ ions are effective in decomposition of uracil molecules. One of the decomposition products, 5,6-dihydro-uracil, was separated by high performance liquid chromatograph (HPLC) and detected using an UV-light detector. Its yield increases first but then decreases as the ion dose increasing. In addition, the mechanism of uracil decomposition and 5,6-dihydro-uracil formation was also discussed.

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Twice investigations around Hangzhou area show that ⁷Be content average in the ground air is 5.9 mBq·m^-3. The content of ⁷Be is the highest in autunm-winter period reaching about 7.7mBq·m^-3; the next is in spring about 6.2mBq•m^-3; then is in the early summer about 5.7 mBq·m^-3, close to the annual average level; and the lowest in a year is in summer-autumn period with a value about 3.8 mBq•m^-3. Exhibited is a decreasing trend from autumn-winter period to summer-autumn of the next year, which is negatively correlated with the variation of the seasonal rainfall in Hangzhou area. But this trend is different from that reported by UNSCEAR: it is the highest in spring and the lowest in the late autumn, which is based only on ⁷Be falling down from the stratosphere. However, the present investigation shows that the seasonal rainfall is the main factor influencing the variation trend of ⁷Be content in the air.

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