⁶⁷Ga-EDTMP was synthesized in a single step by adding ⁶⁷GaCl₃ to EDTMP solution. Dependences of the radiolabeling yield of ⁶⁷Ga-EDTMP on EDTMP concentration, pH and reaction time were examined. Under the optimum conditions, the radiolabeling yield of ⁶⁷Ga-EDTMP was more than 97%. A biodistribution experiment in mice showed that ⁶⁷Ga-EDTMP was mainly absorbed by skeleton and reached 13.25% at 0.5 h after injecting, then kept a high level in 72 h with a maximum value of 16.82% at 48 h. The results suggest that ⁶⁷Ga-EDTMP might be a potential bone pain palliation radiopharmaceutical due to its high skeletal uptake, rapid blood clearance and relatively low soft-tissue absorption. But further work must be done to determine whether ⁶⁷Ga-EDTMP is useful in the treatment of painful osseous metastases.