Studies on apoptosis in bone tumor cells induced by 153Sm

RADIOCHEMISTRY, RADIOPHARMACEUTICALS AND NUCLEAR MEDICINE

Studies on apoptosis in bone tumor cells induced by ¹⁵³Sm

ZHU Shou-peng，

XIAO Dong，

HAN Xiao-feng

Nuclear Science and Techniques

Vol.15, No.2

pp.106-110

Published in print 01 Apr 2004

CLC：R817.8,R738.1

22100

The apoptosis in human bone tumor cells induced by internal irradiation with ¹⁵³Sm was studied. The morphological changes in bone tumor cells were observed by electronic and fluorescent microscopy, as well as DNA agarose gel eletrophoresis. DNA chain fragmentation, microautoradiographic tracing and the inhibition rate of proliferation in bone tumor cells exposed to ¹⁵³Sm with different duration time were examined. It was demonstrated that the bone tumor cells exposed to ¹⁵³Sm displayed nuclear fragmentation, pyknosis, margination of condensed chromatin, and formation of membrane bounded apoptotic bodies, whereas the percentage of DNA chain fragmentation of bone tumor cells increases in direct proportion to the duration of irradiation with ¹⁵³Sm, as well as DNA ladder formation in apoptotic cells. Also a marked inhibition effect of proliferation in bone tumor cells after exposure with ¹⁵³Sm was observed.

ApoptosisBone tumer cells153SmNuclear fragmentationDNA ladder formation

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