DNA gel electrophoretic and microaut oradiographic studies on apoptosis in bone tumor cells after exposure with 153Sm-EDTMP

DNA gel electrophoretic and microaut oradiographic studies on apoptosis in bone tumor cells after exposure with ¹⁵³Sm-EDTMP

ZHU Shoupeng，

XIAO Dong，

HAN Xiaofeng

Nuclear Science and Techniques

Vol.10, No.1

pp.5-7

Published in print 01 Feb 1999

24500

The apoptosis in bone tumor cells is studied after ¹⁵³Sm-EDTMP irradiation. Fragmented DNA is analyzed by agarose gel electrophoresis. Experimental observations show that ¹⁵³Sm-EDTMP exposure induces the internucleosomal DNA damage in bone tumor cells the DNA ladder pattern formation in bone tumor cells is shown. At the same time, the microau-toradiographic study indicates that ¹⁵³Sm-EDTMP could permeate through cell membrane and displays membrane-seeking condensation in bone tumor cells. Soon afterwards ¹⁵³Sm-EDTMP could be phagocytized by the tumor cells and distributed in cytoplasm as well as nucleus in the form of phagosome. With the prolongation of observing time, the membrane-bounded apoptotic bodies are observed.

DNA gel electrophoresisMicroautoradiographyApoptosisBone tumor cellsIrradiation153Sm-EDTMP

References

Billings E, Hosain F. Clin Nucl Med, 1990, 15(2): 364

Nielsen O S, Munro A J, Tannock I F et al. J Clin Oncol, 1991, 9(3): 509

Coyle N, Adelhardt J, Foley K M et al. J Pain Sympt Manage, 1990, 5(1): 83

ZHU Shoupeng. Radiotoxicology, 2nd ed. Beijing: Atomic Energy Press, 1992. 504

ZHU Shoupeng, LAI Guanhua, WANG Liuyi. Nucl Sci Tech, 1994, 5(2): 93

ZHOU Shoupeng. Autoradiographic tracing. Beijing: Atomic Energy Press, 1995. 100

ZHOU Shoupeng, Radiotoxicology of prome-thium. Beijing: Atomic Energy press, 1994. 12

Akagi Y, Ito K, Sawada S. Int J Radiat Biol, 1993, 64(1): 47