SOFT X-RAY IMAGES OF CHROMOSOMES WITHIN INTACT TUMOR CELLS TREATED WITH COLCHICINE

Wei Daoyan，

Shen Hengjia，

Xu Wenxuan，

Tang Esheng

Nuclear Science and Techniques

Vol.3, No.4

pp.244-247

Published in print 01 Nov 1992

49800

In order to avoid any possible effect of separating procedures from intact cell on morphologic structure of the chromosomes, cultivated Hep-2 tumor cells were treated with colchicine and observed by soft X-ray contact microscopy for the first time. The fine structures of chromosomes are more clear with stereo features. The thread-like and coarse granular structures twine and tangle up together within chromosome masses which can not easily be revealed in Wright's stained sample by light microscope or osmium stained sample by transmission electron microscope

SoftX-ray contact microscopyTumor cellsColchicineChromosomes

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