An INAA technique was applied to determine simultaneously abundances of rare-earth, transitional metal, large-ion lithophile and high field strength elements in volcanic rocks from Tangbale ophiolite belt. The detailed study on trace element geochemistry shows that the volcanic rocks were erupted in the back-arc basin. The volcanic rocks of early and middle stages of the expanding period of the basin have low REE and other incompatible element contents. At early and late stages of closing period of the basin, alkalic basalts, basaltic andesites and andesites were erupted in which light REE and other incompatible elements were enriched.