Chromatin and DNA chain fragmentation studies on apoptosis in immune cells induced by 235U and radioprotection of IL-2 or IL-6

Chromatin and DNA chain fragmentation studies on apoptosis in immune cells induced by ²³⁵U and radioprotection of IL-2 or IL-6

Zhu Shou-Peng，

Zhang Lan-Sheng，

Fu Qiang

Nuclear Science and Techniques

Vol.9, No.1

pp.49-51

Published in print 01 Feb 1998

34300

The apoptosis in human acute lymphoblastic leukemia cell line Molt-4 cell and macrophage cell line Ana-1 cell are studied after internal irradiation with enriched uranium ²³⁵U. The cumulative absorption dose of ²³⁵U in cultural cells through different periods are estimated. The fluorescence microscopic observations indicate that Molt-4 and Ana-1 immune cells internally irradiated by ²³⁵U displayed significant chromatin fragmentation and marked pyknosis in immune cells nuclei, as well as DNA chain fragmentation and apoptotic bodies formation. It should be noted that DNA chain fragmentation induced by ²³⁵U may be inhibited statistically by IL-2 (interleukin-2) or IL-6 treatment.

ChromatinDNA chain fragmentationApoptosisMolt-4 cellAna-1 cellEnriched uraniumIL-2IL-6

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